Development and clinical validation of a multiplex PCR-based targeted next-generation sequencing panel for simultaneous DNA/RNA pathogen detection in respiratory tract infections: a comprehensive performance evaluation.
2026-11-12, Clinical chemistry and laboratory medicine (10.1515/cclm-2026-0540) (online)Juan Long, Xiaoyan Tao, Junli Wang, Shenhui Yang, Dandan Tie, Li Jiang, and Jie Zhang (?)
Lower respiratory tract infections (LRTI) remain a leading cause of global morbidity and mortality, with approximately 62 % of cases lacking definitive etiological diagnoses via conventional methods. This study aimed to develop and validate a multiplex PCR-based targeted next-generation sequencing (mp-tNGS) panel for comprehensive pathogen detection in respiratory samples.
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