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Three-dimensional voltage imaging in live larval zebrafish brains using fully genetically encoded voltage indicator.

2026-06-16, Scientific Reports (10.1038/s41598-026-58107-8) (online)
Eun-Seo Cho, Minho Eom, Shihao Zhou, Gyuri Kim, Soi Kim, Cheol-Hee Kim, Kiryl D Piatkevich, and Young-Gyu Yoon (?)
Voltage imaging has emerged as a powerful tool for recording membrane potential changes in living cells, offering a direct measurement of rapid neuronal events with high temporal precision. Since the brain is a three-dimensional circuit, it is essential to record signals across a volume. However, achieving effective three-dimensional voltage imaging over large neuronal populations remains challenging due to the need for high imaging speed, high signal-to-noise ratio, and extensive volume coverage. In this study, we demonstrate in vivo three-dimensional voltage imaging in larval zebrafish using oblique plane microscopy and QFDBD-QUAS-driven expression of the genetically encoded voltage indicator Ace-mNeon2-Kv2.1, achieving volumetric imaging rates of up to 200 volumes per second (VPS). This approach enables dye-free voltage imaging, simplifying experimental workflows and improving the reproducibility of in vivo voltage imaging experiments for investigating neuronal circuit dynamics in the living zebrafish animal model.
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